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These cells include cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, classically activated macrophages (M1 macrophages), and helper T cells (Th cells) ( With the advancement of single-cell sequencing technology, the roles of T cells in anti-tumor immune responses differ across various subtypes ( The synergistic actions of these cells constitute the body’s first line of defense against tumor growth and metastasis